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Analysis of Variance (ANOVA) is a hypothesis test that is used to simultaneously compare the population means of three or more groups.

 

An application-oriented question on the topic along with responses can be seen below. The best answer was provided by Johanan Collins and Sai Kotari.

 

Applause for all the respondents - Sai Kotari, Hirak Raval, Johanan Collins, Sanchita Roy, Afzal Wadood.

Featured Replies

Q 439. ANOVA (Analysis of Variances) is the most popular method to compare more than 2 averages. However, one can only apply ANOVA if certain conditions are met. Explain ANOVA and also highlight the other methods to compare more than 2 averages?

 

Note for website visitors - Two questions are asked every week on this platform. One on Tuesday and the other on Friday.

Solved by Sai Kotari

  • Solution

Parametric analysis of Variance (ANOVA) is used to compare multiple populations and even subgroups of those populations, tells us if there are any statistical differences between the means of three or more independent groups. ANOVA is extension of t-test which allowed us to test two groups if there is difference in means. If you have multiple groups, you could use t-test multiple times but a better way of doing that is through ANOVA.

Anova is the ratio of Variability between and Variability within.

 

Various ways of comparing variances for more than 2 population:

  1. One-way Anova/Single factor: One-way means the analysis of variance has one independent variable, Measures single factor from multiple sources. It helps to identify if there are significant differences between the means of independent variables.
  2.  Factorial ANOVA/Two-Way Anova- Covers ANOVA tests with two or more independent categorical variables. It allows us to “account for variation” for the ROW level due to other factors as each row will have its own mean and std deviation.  This allows greater focus on Group differences making it easier to detect differences. Through this we are attempting to minimize the Error variance by detecting variance.
    • Two-way/Two-factor with replication – Measures 2 factors, but has multiple repetitions of each combinatio
    • Two-way/Two-factor without replication/ Randomized block design - Measures 2 factors

 

Total variance:

Total variance is ratio of between groups by within groups.  Larger the difference, the more likely the groups have different means and that we should reject the Null Hypothesis.

 

a)     Variation within each group

b)     Variation between the groups

 

Concept of confidence interval:

ANOVA tells us groups are different, but it does not tell us what the difference is.  Confidence interval helps us assess the location. Pairwise comparison will lead to Type I error by compounding the error.

The most important statistics in the analysis of variance table are the p-value (P), S, R2, and adjusted R2 values. Collectively, these values tell if means are significantly different from each other

P-value

-        If P is less than or equal to the alpha level, one or more means are significantly different.

-        If P is larger than the a-level, the means are not significantly different.

If the ANOVA results indicate that there are significant differences, we can look at the individual statistics and confidence intervals to learn more about the differences.

S, R2, and adjusted R2 are measurements to show how good the model fits the data.

 

There are multiple comparison methods:

-        Fisher’s LSD – This is used in conjunction with ANOVA. While ANOVA tells us if two of the groups are different from each other, Fisher’s LSD tests two specific groups against each other.

-        Tukey Method – For pairwise comparison after One Factor ANOVA

-        Scheffe Procedure – Similar to Tukey Method, this method is used when Null Hypothesis is rejected after ANOVA, this test is used to determine where the difference exists.

-        Bonferroni Correction – Method used to detect false positives

 

When might you use Anova?  A few scenarios as example when we might want to use Anova:

 

·        A group of patients trying three different therapies and to compare if one therapy is better than the others.

·        Three different types of drinks for a group of people, and compare the reaction time

·        Students from different colleges take the same exam, check if one college outperforms the other

Anova is nothing but Analysis of Variance, We use 1-Sample t-test and 2 sample t-test for comparing difference between 2 means , Comparing means of more than 2 samples ANOVA is used. However, ANOVA does not tell us which mean is better but it tells us that all the sample means are not equal. Based on ANOVA output shorlisted samples can be further tested. Essentially ANOVA tries to find out source of variation, ration of between variation and within variation. Minitab is the simplest method to understand Analysis of Variance based on P-value. There are 2 types of ANOVA one way and two way ANOVA. One way ANOVA is used for one factor benchmark and two way is used for two factor benchmark. ANOVA can be used in many industries like comparison of lead time between outlets, Plant to plant batch release time comparison, Comparison of efficiency across different set up for same factors etc.  However certain Limitation restrict free usage of ANOVA for any condition like experimental Units must be homogeneous, it completely neglects local controls and factors, not suitable for field design experiments, Always required higher sample size, Interpretation of intersections are difficult. Kruskal-Wallis test and Median test can be used for comparison of more than 2 means.

Parametric tests to compare variances for more than two populations are given below

One Independent Variable – 1-Way ANOVA (Completely Randomized Design).

Subjects are assigned randomly to treatments. This design contains only one independent variable with one or more classifications or treatment levels. In this design, the Total Sum of Squares (the variation in the data - SST) is divided into the variance from the treatment (Between Group Variation i.e the columns - SSC) and the error variance (unexplained by the treatment, i.e. within-group variation.  SSE). ANOVA compares the relative size of these two variances using the F Statistic which is the ratio between the two variances.

SST = SSC + SSE

Assumptions for ANOVA. Data is randomly drawn from a normally distributed population and the variance of the populations is equal.

Use of Confidence Intervals.  In the event of the results being significant (p-value<Alpha) the researcher needs to do a post hoc or posteriori test to determine which group is statistically significant from the rest. In order to determine this the Confidence Intervals are plotted and significance is determined by examining the overlap between the confidence intervals.

In addition, Tukey’s Honestly Significant Difference (HSD) test for Equal Sample Sizes, or the Tukey-Kramer Procedure for Unequal Sample Sizes.

Example. The sample consist of the pipe diameter of 4 operators.

image.png.6cc555ed3a610ef3b640d89dbb636d86.pngimage.png.4bbf677bfef96cd16e94dcc7953ddec0.png

The p-value < 0.05 indicates that one or more one operator is statistically significantly different from the other operators.

image.png.b254d25fb122d2813f02c89581ee2639.png

Examining the 95% Confidence Interval of the Operators, it can be observed that the Confidence Interval of Operator 3 does not Overlap with other Operators.

This is also evident from the Fisher Individual 95% Cis and Tukey Simultaneous 95% CIs and the Box Plot. Thus, we can conclude that Operator 3 is significantly different from the other 3 Operators.

image.thumb.png.fc417f2a5c688fbd54f0f61340ad38f4.png

 

Further, the Fisher Individual 95% Cis and Tukey Simultaneous 95% CIs have also bet the Operator 3 is a separate group from the remaining 3 Operators.

image.png.1cdec5c6f973415c47f0a2eb20304cd9.png

image.png.c8763a57f41efaef3349f1c2b74077c0.png

One Independent Variable + 1 Block Variable – Randomized Block Design.

The Randomized Block Design is similar to the Completely Randomized Design; however, it has a second variable, referred to as the Blocking Variable that is used to control for confounded or concomitant variables. These variables are not controlled by the researcher but affect the outcome and hence need to be blocked. The SSE (Error Sum of Squares) is segregated into SSR (Sum of Squares Blocks) and SSE (New Error Sum of Squares)

SST = SSC + SSR + SSE

Repeated Measure Design is a randomized block design in which each block level is an individual item/person and that person/item is measured across all treatments. It helps researchers to determine if the means of three or more measures from the same person are similar or different. The repeated measure ANOVA controls the between-subjects variance by removing it from the error term and measuring it separately. 

ANOVA for Latin Square Design

The Latin Square Design utilizes the Blocking principle. It is used to remove two nuisance sources of variability which are in the rows and columns of the square. The rows, and columns are the two restrictions on randomization. The model is totally additive, in that there is no interaction between the treatment, rows and columns. The Analysis of Variance divides the total sum of squares into the sum of squares for the rows, columns, treatment, and error. The F test ratio of MS(Treatment)/MS(Errors) is used to determine if there is no difference in the treatment means. (MS – Mean Sum of Squares)

Randomized Incomplete Block Design.

When it is not possible to run all the treatment combinations in each block, the randomized incomplete block design is used. When all treatments comparisons are equally important, the Balanced Incomplete Block Design is used. This design ensures that each block is selected in a balanced manner so that any pair of treatments are selected the same number of times as any other pair.

 

Two Independent Variables – Two-Way ANOVA

In this design, two or more independent variables are explored at one time. These are also called factorial designs. In this design, each and every level is studied under the conditions of every level of all other treatments. The design can have 3,4, …, n independent variables being studied at one time. For example, the independent variables can be machines, operators, shifts, day of the week, suppliers, and raw materials. All of these independent variables can be done in one study. This will be a Six-Way ANOVA.

The Completely Randomized Design, the Randomized Block Design, and the n-Way ANOVA all have one dependent variable.

Chi-Square Goodness of Fit Test.

This test is used to analyze the probabilities of multinomial distribution trials along a single direction. For example, to study Education with 4 possible outcomes, viz. illiterate, Primary School, Secondary School, Bachelors's and above, the single dimension is Education and the possible outcomes are the levels of education. Also, it is imperative that on one trial only one outcome can occur. The Chi-Square Goodness of Fit test measures the difference between the observed frequencies and expected frequencies. The Chi-Square Distribution is used to measure the significance. It is a one-tailed test since Chi-Square of Zero means the perfect agreement between observed and expected values.

Chi-Square – Test of Independence.

The Goodness of Fit test cannot be used to measure the analyse two variables at one time. For this purpose, the Test of Independence is done. Test of independence can analyse the frequencies of 2 variables with multiple categories to determine if the two are independent. Use Case. To determine if the type of pizza topping is independent of the customer's age or the citizenship is independent of investment in bitcoin.

Non-Parametric tests to compare variances for more than two populations are given below

One Independent Variable – Kruskal Wallis Test.

This test is the nonparametric equivalent to the one-way ANOVA. It is used to check if 3 or more samples come from the same/similar or different populations. It can be used for Ordinal Data and is not based on the shape of the population distribution. It assumes that the groups are independent of each other and random selection of items in the groups.   

image.png.043f9a6572da6c67a20409b34aaf1a57.png

The Kruskal Wallis Test for the above data shows a p-value of < 0.05 indicating that one of the operators is statistically significant from the others. Examination of the results shows that Operator 3 is significantly different from other operators.

image.png.1a58932b2b8d1b60e9c71c7473689be9.png

One Independent Variable – Mood’s Median Test.

It is used instead of the Kruskal Wallis test when there are outliers present in the data. The results from the Mood’s Median Test indicate a p-value of 0.003 with is significant with an Alpha of 0.05.

Confidence Interval Mood’s Median Test. On observing the 95% Midian Confidence Interval of the three operators, it can be seen that the CI of Operator 3 is distinct from the other operators.

image.png.1230ea3572b08287f24891d9b3056cd7.png

One Independent Variable + 1 Block Variable – Friedman Test

This is the nonparametric equivalent to the randomized block design. If the normality of the data cannot be assumed or the data is ranked the Friedman test is used. It assumes independent blocks; no interaction is present between blocks and treatments, and the observations within each block can be ranked. In the Friedman Test below, the Day of the Week is Blocked. The p-value of 0.007 < Alpha of 0.05 indicates that one of the operators is statistically significantly different. On observation, it can be seen that Operator 3 is different from the rest of the Operators.

image.thumb.png.c35b9ba6371cc3008097a68356fb06b7.png

Multivariate Analysis of Variance (MANOVA). This test is used when there are more than one continuous Dependent Variables. A one-way MANOVA would examine the effect of one independent variable on two dependent variables. For example, to study the effect of education on income and expenditure. A two-way MANOVA would examine the effect of two independent variables on two dependent variables. For example, to study the effect of education and citizenship on income and expenditure.

image.thumb.png.85842491c323723ae12e68b653392929.png

Multivariate Analysis of Covariance (MANCOVA).

This test includes covariates. It involves finding out the statistical significance between multiple dependent variables (continuous) and an independent (grouping) variable and controlling with additional variables called covariates. Covariates reduce the error term hence the effect of the covariate is removed from the relationship of the independent and dependent variables.

image.thumb.png.bd2b620dbc0438aa81d4de6c74ba47ba.png

 

References

https://medium.com/nerd-for-tech/everything-about-manova-and-mancova-4c1c237af464

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Analysis of variance (ANOVA) is used to assess the differences between means of 2 or more groups.

It is a statistical hypothesis test to determine whether the means of at least two populations are different. Conditions for using ANOVA are:

·         a continuous dependent variable (Y) ad and a discrete independent variable (X)

·         should be a normal distribution.

·         Samples must be independent.

·         Population variances must be equal

·         Groups must have equal sample sizes.

 

Hypothesis:

Ho: μ1 = μ2 = μ3 =… μn

Ha: μ1 ≠ μn

 

A significant P value implies a low probability that the mean values for all groups are equal; it only tests for an overall difference between groups. Once the overall significance is arrived at, then we can use multiple comparison procedures for individual group comparisons.

 

The right AVOVA test to perform can be decided basis the number of independent variables that are included in the ANOVA test

A.      One-way means the analysis of variance has one independent variable. Example: AHT of staff at different experience levels like <6 months and > 6 months

B.      Two-way means the test has two independent variables (which can have multiple levels), Example, For Jam sales/week, independent variables of brand of Jams and how many calories it has. Another example: AHT of staff at different experience levels like 3-6 months, 6-12 and >12 months for Different experience background like Insurance, Banking, etc.

o   The results from a Two- Way ANOVA will calculate a main effect and an interaction effect.

o   The main effect is similar to a One -Way ANOVA: each factor’s effect is considered separately and with the interaction effect, all factors are considered at the same time.

C.      MANOVA (multivariate analysis of variant) is another form of ANOVA for several dependent variables. For example, AHT for Task 1 for different Tenure groups/Experience background and AHT for Task 2 as well. Possibly to assess if the combination has any adverse impact on speed of Task 2.

It tests multiple dependent variables at the same time by testing:

a.      changes to the independent variables have statistically significant effects on dependent variables

b.      interactions among dependent variables

c.       interactions among independent variables

For data that is not Normally distributed, we can use a non-parametric, analogue of one-way ANOVA (called Kruskal-Wallis ANOVA)

In many situations , we have to compare central tendencies(generally mean is compared) of multiple samples. For 2 sample, t test is widely used. We can use Z test for large sample size.

ANOVA (Analysis of variance) is used in cases when we have to compare more than 2 means. Though we can use multiple t test but it will be time consuming and less efficient.

ANOVA is a parametric test and requires various assumption for the populations samples to be compared. This includes assumption of Normality, Independence and approximately equal variance. ANOVA is not used in the Nominal data .

Below are the steps to perform the ANOVA:

  1. Calculate the mean of all the samples
  2. Define the null and alternate hypothesis (e.g.: Ho= All samples have equal mean, Ha= All Sample means are not equal)
  3. Perform calculation to get the Sum of squares and mean squares based on degrees of freedom for within and between samples differences.
  4. Calculate the F statistic.
  5. Look up statistical Table , Compare the F statistic with the tabular values and conclude on the results.

 

ANOVA is a parametric test and hence can not be used in all situations. We can use Kruskal Wallis test which is the non parametric alternative to the One Way ANOVA. It compares the sum of ranks in the samples instead of mean.  

Very interesting answers to an equally interesting topic :)

 

There are two winners for this question - Johanan Collins and Sai Kotari. Johanan for the shear vastness of information provided with respect to  ANOVA and Sai for highlighting various related concepts like within and between variations plus confidence intervals.

 

Well done!

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